ApoTome1 inverse BIOTEC (Zeiss)
Our Apotome microscope inverted is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome.
Category
Widefield, ApoTome
Location
BIOTEC, 2nd floor, LMF CMCB, room 225
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
2.5x/0.12 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 8.7 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 202 2.5x/0.12 Zeiss |
| Order Number: | 420120-9901-000 |
| Inventory Number: | 25177461 |
5x/0.12 A-Plan, Air, Ph0, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 10.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 92 5x/0.12 Ph0 Zeiss |
| Order Number: | 441021-9910-000 |
10x/0.45 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 121 10x/0.45 Zeiss |
| Order Number: | 000000-1063-139 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 122 20x/0.8 Zeiss |
| Order Number: | 440640-9903-000 |
40x/0.95 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.25 mm |
| Nomarski Prism: | EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 86 40x/0.95 Zeiss |
| Order Number: | 440654-9902-000 |
63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Nomarski Prism: | Planapo 63x/1,40 III (1122-909) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 38 63x/1.4 Oil Zeiss |
| Order Number: | 000000-1113-108 |
100x/1.45 Achroplan, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.11 mm |
| Nomarski Prism: | alpha Plan-Fluar 100/1,45 III (1131-718) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 97 100x/1.45 Oil Zeiss |
| Order Number: | 000000-1084-514 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI ()
single cube for Alexa 350, DAPI
- BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
- LP 400, dichromatic mirror, longpass, Zeiss
- BP 460/50 (BP 435-485), emission filter, bandpass, Zeiss
Zeiss
GFP (set F46-002)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
NIKON, set F46-002
Cy3 (set 49004)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 545/25 (BP 533-558), excitation filter, bandpass, Chroma
- LP 565, dichromatic mirror, longpass, Chroma
- BP 605/70 (BP 570-640), emission filter, bandpass, Chroma
Zeiss, set 49004
Cy5 (set 49004)
single cube for Alexa633/Cy5
- BP 625/50 (BP 600-650), excitation filter, bandpass
- LP 665, dichromatic mirror, longpass
- BP 690/50 (BP 665-715), emission filter, bandpass
Zeiss
Calibration (set 49004)
single cube for Alexa633/Cy5
Zeiss
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
Camera
Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm
| port | front port |
| magnification | 0.6x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
| Inventory number | 175067 |
| Serial number | 243092653 |
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
| Serial number | 110060309 |
XY Stage
Structured Illumination
ApoTome1, Zeiss
structured illumination for optical sectioning
Computer
Software
Additional Information