Dragonfly CRTD (Andor)
Confocal live cell imaging system for fast and gentle imaging. The system provides 6 laser lines ranging from 405-637nm, Air, W and Si objectives, a spinning disc unit with two pinhole discs, a zoom in function for maximum laser power on a smaller area of the sample and an additional magnification (1x, 1,5x or 2x) in front of the sCMOS camera. The incubation allows temperature, CO2 and humidity control. The stage is motorized for multi-position and mosaic applications, the microscope z-drive and the 150µm range fast Piezo might be used. The system can be run via Fusion software or ANDOR IQ.
Category
Spinning disk
Location
CRTD, basement south, LMF CMCB, room -1.121
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
4x/0.16 U Plan SApo, Air, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 13 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 203 4x/0.16 Olympus |
| Order Number: | UPLSAPO4X |
10x/0.4 U Plan SApo, Air, DIC, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 3.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 197 10x/0.4 Olympus |
| Order Number: | N2249100 |
20x/0.75 U Plan SApo, Air, DIC, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.6 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 198 20x/0.75 Olympus |
| Order Number: | N1480500 |
30x/1.05 U Plan SApo, Silicone, DIC, OLYMPUS
| Immersion: | Silicone |
| Collar: | temperature and coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.92 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 196 30x/1.05 Sil Olympus |
| Order Number: | N4212100 |
| Inventory Number: | 0000test1234 |
40x/1.15 U Plan SApo, Water, DIC, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.25 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 199 40x/1.15 W Olympus |
| Order Number: | N2709300 |
60x/1.3 U Plan SApo, Silicone, DIC, OLYMPUS
| Immersion: | Silicone |
| Collar: | temperature and coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.3 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 204 60x/1.3 Sil Olympus |
| Order Number: | UPLSAPO60xS2 |
60x/1.2 U Plan SApo, Water, DIC, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 205 60x/1.2 W Olympus |
| Order Number: | UPLSAPO60XW |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI ()
single cube for DAPI, Alexa 350
- BP 377/50 (BP 352-402), excitation filter, bandpass, OLYMPUS
- LP 409, dichromatic mirror, longpass, OLYMPUS
- BP 447/60 (BP 417-477), emission filter, bandpass, OLYMPUS
OLYMPUS
GFP (set 49002)
single cube for Alexa488/GFP
- BP 470/40 (BP 450-490), excitation filter, bandpass, OLYMPUS
- LP 495, dichromatic mirror, longpass, OLYMPUS
- BP 525/50 (BP 500-550), emission filter, bandpass, OLYMPUS
OLYMPUS, set 49002
Dsred (set 49005)
single cube for DsRed
- BP 545/30 (BP 530-560), excitation filter, bandpass, OLYMPUS
- LP 570, dichromatic mirror, longpass, OLYMPUS
- BP 620/60 (BP 590-650), emission filter, bandpass, OLYMPUS
OLYMPUS, set 49005
CFP (set 49005)
single cube for CFP
- BP 438/24 (BP 426-450), excitation filter, bandpass, OLYMPUS
- LP 458, dichromatic mirror, longpass, OLYMPUS
- BP 483/31 (BP 467-498), emission filter, bandpass, OLYMPUS
OLYMPUS
YFP (set 49005)
single cube for YFP
- BP 500/24 (BP 488-512), excitation filter, bandpass, OLYMPUS
- LP 520, dichromatic mirror, longpass, OLYMPUS
- BP 542/22 (BP 531-553), emission filter, bandpass, OLYMPUS
OLYMPUS
Additional Filters
DAPI, GFP, RFP, Cy5, CFP, YFP, CFP, YFP, Alexa 488, eGFP, FITC, RFP, TRITC, GFP, RFP, Cy5, Alexa 647, Alexa 680
- (EM 440+521+561+640), emission filter, quadro bandpass, Andor
- BP 480/40 (BP 460-500), emission filter, bandpass, Andor
- BP 540/30 (BP 525-555), emission filter, bandpass, Andor
- (EM 442+514), emission filter, double bandpass, Andor
- BP 525/50 (BP 500-550), emission filter, bandpass, Andor
- BP 600/50 (BP 575-625), emission filter, bandpass, Andor
- (EM 488+561), emission filter, double bandpass, Andor
- BP 700/75 (BP 663-738), emission filter, bandpass, Andor
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
405nm
Laser Diode, 100mW, integrated
445nm
Laser Diode, 75mW, integrated
488nm
Laser Diode, 50mW, integrated
514nm
Laser Diode, 40mW, integrated
561nm
Laser Diode, 50mW, integrated
637nm
Laser Diode, 140mW, integrated
U-HGLGPS
OLYMPUS, Mercury arc lamp, 130W, integrated
Camera
Andor Zyla PLUS sCMOS, Monochrome sCMOS camera, dexel size 6.5 µm
| port | left side port |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
XY Stage
Scan Head
Incubation
Computer
Software
Additional Information