SP5 II upright BIOTEC (Leica)
Our Leica TCS SP5 II confocal setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
Category
Laser scanning confocal
Location
CRTD, basement south, LMF CMCB, room -1.121
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
20x/0.7 HC PL APO CS, Air, DIC, Leica
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.59 mm |
| Nomarski Prism: | C |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 09 20x/0.7 Leica |
| Order Number: | 11506513 |
20x/0.5 HC APO L, Water, DIC, Leica
| Immersion: | Water |
| Working Distance: | 3.5 mm |
| Nomarski Prism: | D1 |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 14 20x/0.5 Water dipping Leica |
| Order Number: | 11506147 |
40x/1.25 HC PL APO, Oil, DIC, Leica
| Immersion: | Oil |
| Collar: | NA correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.1 mm |
| Nomarski Prism: | D |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 20 40x/1.25-0.75 Oil Leica corr. |
| Order Number: | 11506250 |
63x/1.4 HC PL APO, Oil, DIC, Leica
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.1 mm |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 51 63x/1.4 Oil Leica |
| Order Number: | 11506187 |
63x/1.2 HC PL APO lambda blue, Water, DIC, Leica
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.22 mm |
| Nomarski Prism: | D |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 59 63x/1.2 Water Leica corr. |
| Order Number: | 11506213 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set A)
single cube for Alexa 350, DAPI
- BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
- LP 400, dichromatic mirror, longpass, Leica
- LP 425, emission filter, longpass, Leica
Leica, set A
GFP (set I3)
single cube for Alexa488/GFP, FITC
- BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
- LP 505, dichromatic mirror, longpass, Leica
- BP 527/30 (BP 512-542), emission filter, bandpass, Leica
Leica, set I3
Cy3 (set N2.1)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
- LP 585, dichromatic mirror, longpass, Leica
- LP 590, emission filter, longpass, Leica
Leica, set N2.1
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Leica, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Leica, Metal halide lamp, 120W, integrated
405nm pulsed
Laser Diode, integrated
458, 477, 488, 496, 514nm multiline
Laser Argon, integrated
561nm
Laser DPSS, integrated
633nm
Laser HeNe, integrated
XY Stage
Scan Head
Incubation
Computer
Software
Additional Information