Biopolis Dresden Imaging Platform

SP5 MP inverse CRTD (Leica)

Our Leica SP5 MP is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. A Mai Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes. A resonating scanner allows fast confocal imaging.

Category

Laser scanning confocal, Multi photon

Location

CRTD, basement south, LMF CMCB, room -1.119

Suitable for Specimen
Live samples
Fixed samples
Tissue sections
Suitable for Technique
Spectral unmixing
Microscope Stand

Leica DMI 6000, inverted stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Leica SuperZ stage

Objectives

10x/0.4 HC PL APO CS, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2.2 mm
Nomarski Prism: A
Thread: M25
Tube Length: infinity
Objective ID: CMCB 28 10x/0.4 Leica
Order Number: 11506285

20x/0.7 HC PL APO CS, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.59 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 46 20x/0.7 Leica
Order Number: 11506166

25x/0.95 HC IRAPO L, Water, DIC, Leica

Immersion: Water
Coverslip Thickness: 0.17
Working Distance: 2.4 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 153 25x/0.95 Water Leica
Order Number: 11506340

40x/0.75 HC PL APO, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.28 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 15 40x/0.75 Leica
Order Number: 11506173

40x/1.25 HC PL APO lambda blue, Oil, DIC, Leica

Immersion: Oil
Collar: NA correction
Coverslip Thickness: 0.17
Working Distance: 0.1 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 30 40x/1.25-0.75 Oil Leica corr.
Order Number: 11506253

63x/1.2 HC PL APO, Water, DIC, Leica

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.22 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 21 63x/1.2 Water Leica corr.
Order Number: 11506279

100x/1.4 HC PL APO CS, Oil, DIC, Leica

Immersion: Oil
Collar: NA correction
Coverslip Thickness: 0.17
Working Distance: 0.22 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 07 100x/1.4-0.7 Oil Leica corr.
Order Number: 11506210
Condenser

BF, DIC I, DIC II, DIC III
NA 0.55, working distance 28 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI (set A)

single cube for DAPI, Alexa 350

  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica

Leica, set A

GFP (set I3)

single cube for Alexa488/GFP

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Leica
  • LP 510, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, bandpass, Leica

Leica, set I3

Cy3 (set N2.1)

single cube for Alexa555/Cy3/RFP, TRITC

  • (BP 515-560), excitation filter, bandpass, Leica
  • LP 580, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, longpass, Leica

Leica, set N2.1

polarizer (set Analysator)

polarizer cube for DIC/Polarization

Leica, set Analysator

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Leica, Metal halide lamp, 120W, integrated

405nm pulsed

Laser Diode, integrated

458, 477, 488, 496, 514nm multiline

Laser Argon, integrated

561nm

Laser DPSS, integrated

633nm

Laser HeNe, integrated

Vision II 680-1080nm

Coherent, Laser Multiphotonen Chameleon, integrated
XY Stage

motorized stage

Märzhäuser Type: EK 127x83 mot, Article-No: 31-27-313-0000 / 11522068, SN: 08102455

Scan Head

SP5

conventional+resonant scanner, resonant scanner speed 8 kHz

Internal detectors:

  • 2 spectral detection HyD
  • 3 confocal PMT
  • T-PMT
Incubation

splitable cage incubator

LSI

Feature:

  • CO2

  • Heating

  • Humidity

Computer

HP Z600 Workstation

Windows 7 Professional (64 bit) operating system

  • 3 x 4 GB RAM
  • Intel Xeon X5650 CPU
  • 30" display
  • SP5MP-INV-CRTD
Software

Leica LAS AF, V 2.7.3

  • 1122000, 1134305
  • 3D
  • Acquisition
  • Base
  • Colocalization
  • Dye Finder
  • FRAP
  • FRET
  • Live Data Mode
Additional Equipment

A Mai Tai IR laser allows the use of mutli-photon imaging, for thick or sensitive samples, or UV-excitable dyes.

A resonating scanner allows fast confocal imaging.

Additional Information
  • Inventory Number 71928
  • SIP Number SN: 5100000461
  • Contact for Service Hr. Schreiner (0172/9401123)
  • DRESDEN concept ID 1303