Biopolis Dresden Imaging Platform

WF Olympus upright BIOTEC (OLYMPUS)

Our Olympus videomicroscope upright is designed for routine work on fixed sample. Fluorescent samples and stained histological samples can be imaged due to the use of two possible cameras, a b/w Photometrics and a color Spot CCD. Timelapse, along with z-stacks can be performed with the use of motorized z-stage.

Booking System
Category

Widefield

Location

BIOTEC, 2nd floor, LMF CMCB, room 227

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
3D imaging
Microscope Stand

OLYMPUS BX 61, upright stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Prior NanoScanZ

Objectives

4x/0.13 U Plan Fl, Air, DF, OLYMPUS

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 17 mm
Nomarski Prism: 10x/0.30W II (444438)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 73 4x/0.13 Olympus
Order Number: 1234
Inventory Number: 0987

10x/0.4 U Plan SApo, Air, DIC, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 3.1 mm
Tube Length: infinity
Objective ID: CMCB 70 10x/0.4 Olympus

20x/1.35 U Plan Fl, Air, Ph1, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2.1 mm
Tube Length: infinity
Objective ID: CMCB 71 20x/0.5 Ph1 Olympus

20x/0.85 U Plan SApo, Oil, DIC, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.17 mm
Tube Length: infinity
Objective ID: CMCB 90 20x/0.85 Oil Olympus

40x/0.95 U Plan SApo, Air, DIC, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.18 mm
Tube Length: infinity
Objective ID: CMCB 72 40x/0.95 Olympus

40x/1.3 U Plan FL N, Oil, DIC, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Tube Length: infinity
Objective ID: CMCB 191 40x/1.3 Oil Olympus
Order Number: N1478700

60x/1.35 U Plan SApo, Oil, DIC, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.15 mm
Tube Length: infinity
Objective ID: CMCB 74 60x/1.35 Oil Olympus

100x/1.35 U Plan Apo, Oil, Ph3, OLYMPUS

Immersion: Oil
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Nomarski Prism: PA 100/1,4 HC II (426949)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 69 100x/1.35 Oil Ph3 Olympus
Condenser

BF, DF, DIC I, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI ()

single cube for Alexa 350, DAPI

  • BP 365/10 (BP 360-370), excitation filter, bandpass, OLYMPUS
  • LP 400, dichromatic mirror, longpass, OLYMPUS
  • LP 420, emission filter, longpass, OLYMPUS

OLYMPUS

GFP (set 49002)

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

OLYMPUS, set 49002

GFP (set 49002)

single cube for Alexa488/GFP, FITC

  • BP 480/20 (BP 470-490), excitation filter, bandpass
  • LP 495, dichromatic mirror, longpass
  • BP 525/50 (BP 500-550), emission filter, bandpass

OLYMPUS

Cy3 (set 49004)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 545/25 (BP 533-558), excitation filter, bandpass, Chroma
  • LP 565, dichromatic mirror, longpass, Chroma
  • BP 605/70 (BP 570-675), emission filter, bandpass, Chroma

OLYMPUS, set 49004

TRITC (set 49004)

single cube for Alexa555/Cy3/RFP, Cy3

  • BP 540/20 (BP 530-550), excitation filter, bandpass, OLYMPUS
  • LP 570, dichromatic mirror, longpass, OLYMPUS
  • LP 590, emission filter, longpass, OLYMPUS

OLYMPUS

polarizer (set 49004)

single cube for DIC/Polarization

OLYMPUS

Cy5 (set 49006)

single cube for Alexa633/Cy5

  • BP 620/60 (BP 590-650), excitation filter, bandpass, AHF
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss

OLYMPUS, set 49006

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HBO

Mercury arc lamp, 100W
Camera

Coolsnap FX, Monochrome CCD camera, dexel size 6.7 µm

port top port
magnification 1x
bit depth 12 bit
sensor size 8.7 x 6.9 mm
maximum frame size 1300 x 1030 dexel
maximum full chip frame rate 15.38 fps
Inventory number 106554000
Serial number A01L86004

Spot insight color IN1420, Color CCD camera, dexel size 7.4 µm

port top port
magnification 1x
bit depth 14 bit
sensor size 15.2 x 15.2 mm
maximum frame size 2048 x 2048 dexel
maximum full chip frame rate 5 fps
Inventory number 138791000
Serial number 239504

Olympus DP71, Color CCD camera, dexel size 6.45 µm

port top port
magnification 1x
bit depth 12 bit
sensor size 8.7 x 6.6 mm
maximum frame size 4080 x 3072 dexel
Inventory number 170556000
Serial number 6H06054
XY Stage

manual stage

Olympus U-UCD8A, SN: 1E05676 with

  • fixed holder for 4 slides
Computer

DELL Precision WorkStation 380

Windows XP Professional operating system

  • 2 x 1 GB + 2 x 0.5 GB RAM
  • Intel Pentium 4 CPU
  • NVIDIA QUADRO FX540 grafic card
  • 19" display
  • Olympus-upr-BIOTEC
Software

Molecular Devices MetaXpress,

  • test1234567890

Molecular Devices MetaMorph,

  • test1234567890
  • 3D

Diagnoscics Instruments SPOT Basic Software, V 5.1, 5678

  • test1234567890
Additional Equipment

Gibt es keins.

Additional Information
  • Inventory Number 106553000
  • SIP Number 1223334444
  • Contact for Service Hr. Hans-Jürgen Engelmann (0170/7079007)
  • Service Contract Number XYZ 12345
  • DRESDEN concept ID 1307
  • Date of Installation 14.08.2018
  • End of Warranty 14.08.2020
  • Service Visits unzählige ...