Biopolis Dresden Imaging Platform

WF Screening System BIOTEC (Molecular Devices)

t is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates.

Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed..

Category

Widefield, High throughput microscope

Location

BIOTEC, 2nd floor, LMF CMCB, room 242

Suitable for Specimen
Live samples
Fixed samples
Suitable for Technique
Multiple positions
Multi wellplate imaging
Microscope Stand

Molecular Devices ImageXpress Micro XL, inverted stand
motorized objective revolver, motorized z-focus

Objectives

2x/0.1 CFI PlanApo Lambda, Air, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 8.5 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 184 2x/0.1 Nikon
Order Number: 1-6300-0451

4x/0.2 CFI PlanApo Lambda, Air, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 20 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 185 4x/0.2 Nikon
Order Number: 1-6300-0121

10x/0.4 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 4 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 186 10x/0.45 Nikon
Order Number: 6500-0120

20x/0.75 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 1.0 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 187 20x/0.75 Nikon
Order Number: 1-6300-0196

40x/0.95 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.25 - 0.16 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 188 40x/0.95 Nikon
Order Number: 1-6300-0412

10x/0.3 CFI PlanFluor, Air, DIC, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 16 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 189 10x/0.3 Nikon

20x/0.45 CFI S PlanFluor ELWD ADM, Air, Ph1, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 8.2 - 6.9 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 190 20x/0.45 Ph1 Nikon
Inventory Number: test234567890
Condenser

BF, Ph0, Ph1
manual

Transmitted Illumination

(Nikon 100W Pillar Diascopic Illuminator)

Fluorescence Illumination

Spectra X Light engine

Lumencor, LED, integrated
Camera

Array

XY Stage

motorized stage

special stage with

  • multiwell plate holder
Incubation

cage incubator

heating unit from Nikon, Molecular Devices

Feature:

  • Heating

  • CO2

Computer

Windows 7 operating system

Software

Molecular Devices MetaXpress,

  • test1234567890
Additional Information
  • Inventory Number 25007452
  • Contact for Service Hr. Christian Holz (0162/2565931)
  • DRESDEN concept ID 1380