Biopolis Dresden Imaging Platform

CZ5 - Zeiss LSM 700 inverted (Zeiss)

Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ.The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Location

MPI-CBG, LMF MPI-CBG, room 038A

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 46 10x/0.45 Zeiss
Order Number: 420640-9900
Inventory Number: 4064040000

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 47 20x/0.8 Zeiss
Order Number: 420650-9901

25x/0.8 LCI Plan-Neofluar, W / Glyc / Oil, DIC, Zeiss

Immersion: W / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: 0-0.17
Working Distance: 0.21 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 25/0,8 II (426947)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 41 25x/0.8 Zeiss
Order Number: 420852-9972

40x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 49 40x/1.2 Zeiss
Order Number: 421767-9970
Inventory Number: 4075700000

63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss

Immersion: W / Glyc
Collar: immersion correction
Coverslip Thickness: 0.15-0.18
Working Distance: 0.17 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 50 63x/1.3 Zeiss
Order Number: 420882-9970
Inventory Number: 4092770000

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: PA 63/1,4 HC II (426952), PA 63/1,40 III (426957)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 44 63x/1.4 Zeiss
Order Number: 420782-9900
Inventory Number: 4086040000
Condenser

Fluorescence Filter Cubes

motorized revolver

GFP ()

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 510, dichromatic mirror, longpass, Zeiss
  • BP 540/50 (BP 515-565), emission filter, bandpass, Zeiss

Alexa 555 ()

single cube for Alexa 555, TRITC

  • BP 546/12 (BP 540-552), excitation filter, bandpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • BP 607/65 (BP 575-640), emission filter, bandpass, Zeiss

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 488049-9901-000

Cy5 (set 488049-9901-000)

single cube for Alexa633/Cy5

  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

, set 488049-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120W

Zeiss, Metal halide lamp

405nm

Laser Diode, integrated

488nm

Laser DPSS, integrated

555nm

Laser DPSS, integrated

639nm

Laser DPSS, integrated
XY Stage

motorized stage

Märzhauser Wetzler GmbH EK 130X85 mot. Tango CZ EMV with

  • universal disholder
  • multiwell plate holder
Scan Head

LSM 700

conventional scanner

Internal detectors:

  • 2 confocal PMT (filter)
Extra Photo Manipulation

Zeiss LSM 700

Photo Manipulation light source:

Laser Diode 405nm

Laser DPSS 488 nm

Laser DPSS 555nm

Laser DPSS 639nm

Additional Information
  • See manual
  • Laser power values
  • Contact for Service Zeiss services service.lm.microscopy.de@zeiss.com
  • DRESDEN concept ID 1276