LZ2 - Zeiss Lightsheet Z.1 (Zeiss)
Single Plane Illumination Microscope
The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions.
Suitable for Live imaging of larger specimen in 3D and 4D imaging of larger cleared samples.
Category
Light sheet
Location
MPI-CBG, LMF MPI-CBG, room 356
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.16 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 18.5 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 330 5x/0.16 Zeiss - Detection |
| Order Number: | 420330-8210 |
5x/0.1 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | Mpi-CBG 377/378 5x/0.1 Zeiss - Illumination |
10x/0.2 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | MPI-CBG 379/380 10/0.2 Zeiss - Illumination |
10x/0.2 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | MPI-CBG 381/382 10/0.2 Zeiss - Illumination |
10x/0.2 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0-0.17 |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 288/289 10x/0.2 Zeiss - illumination |
10x/0.2 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | - |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 261 10x/0.2 Zeiss - Illumination |
| Order Number: | 400900-9000 |
10x/0.2 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | - |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 262 10x/0.2 Zeiss - Illumination |
| Order Number: | 400900-9000 |
5x/0.16 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 18.5 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 283 5x/0.16 Zeiss - Detection |
| Order Number: | 420330-8210 |
10x/0.5 Plan-Apochromat, Water, Zeiss
| Immersion: | Water |
| Coverslip Thickness: | - |
| Thread: | M27 |
| Objective ID: | MPI-CBG 373 10/0.5 W Zeiss - Detection |
20x/1 Plan-Apochromat, Water, Zeiss
| Immersion: | Water |
| Collar: | refractive index correction |
| Working Distance: | 2.4 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 329 20x/1.0 W Zeiss - Detection |
| Order Number: | 421452-9700 |
| Inventory Number: | 4909000073 |
20x/1 Plan Apochromat, Water, Zeiss
| Immersion: | Water |
| Coverslip Thickness: | - |
| Thread: | M27 |
| Objective ID: | MPI-CBG 374 20/1.0 W Zeiss - Detection |
20x/1 Plan Apochromat, Clarity - 1.45, Zeiss
| Immersion: | Clarity - 1.45 |
| Thread: | M27 |
| Objective ID: | MPI-CBG 375 20/1.0 Clarity Zeiss - Detection |
20x/1 Plan-Apochromat, Scale - 1.38, Zeiss
| Immersion: | Scale - 1.38 |
| Coverslip Thickness: | - |
| Thread: | M27 |
| Objective ID: | MPI-CBG 376 20x/1.1 Scale Zeiss - Detection |
Fluorescence Filter Cubes
motorized revolver
Full mirror ()
single cube for Full reflection
- mirror
Zeiss
Universal Blue/Green ()
image splitter cube for DAPI, Alexa488/GFP, Alexa555/Cy3/RFP, Alexa633/Cy5
- LP 490, dichromatic mirror
- LP 505, emission filter
- SP 490, emission filter
Zeiss
Universal Green/Red ()
image splitter cube for Alexa488/GFP, Alexa555/Cy3/RFP
- LP 560, dichromatic mirror, longpass
- emission filter
- LP 585, emission filter, longpass
Zeiss
GFP/Cy5 ()
image splitter cube for Alexa488/GFP, Alexa633/Cy5
- LP 580, dichromatic mirror, longpass
- BP 525/40, emission filter, bandpass
- LP 660, emission filter, longpass
Zeiss
Cy3/Cy5 ()
image splitter cube for Alexa555/Cy3/RFP, Alexa633/Cy5
- BP 595/40, emission filter, bandpass
- LP 640, dichromatic mirror, longpass
- LP 660, emission filter, longpass
Zeiss
Universal Green/Far-Red ()
image splitter cube for Alexa488/GFP, Alexa 405, Alexa633/Cy5
- LP 640, dichromatic mirror, longpass
- emission filter
- LP 660, emission filter, longpass
Zeiss
motorized revolver
BFP/Cy5
image splitter cube for Alexa488/GFP, Alexa 405, Alexa633/Cy5
- BP 445/50, emission filter, bandpass
- LP 490, dichromatic mirror
- LP 660, emission filter, longpass
Zeiss
CFP/YFP
image splitter cube for Alexa488/GFP, Alexa 405, Alexa633/Cy5
- BP 480/40, emission filter, bandpass
- LP 510, dichromatic mirror, longpass
- BP 545/40, emission filter, bandpass
Zeiss
FITC/TRITC
image splitter cube for Alexa488/GFP, Alexa 405, Alexa633/Cy5
- LP 560, dichromatic mirror, longpass
- BP 517/25, emission filter, bandpass
- BP 595/40, emission filter, bandpass
Zeiss
Cy5
image splitter cube for Alexa633/Cy5
- LP 640, dichromatic mirror, longpass
- empty
- LP 640, emission filter, longpass
Zeiss
Transmitted Illumination
LED (LED)
Fluorescence Illumination
405nm
Laser Diode, integrated
445nm
Laser DPSS
488nm
Laser DPSS, integrated
514nm
Laser DPSS
561nm
Laser DPSS, integrated
638nm
Laser DPSS
Camera
PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm
| port | top port |
| bit depth | 16 bit |
| sensor size | 13.3x13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 30 fps |
PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm
| port | left side port |
| bit depth | 16 bit |
| sensor size | 13.3x13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 30 fps |
Incubation
Additional Information