W7 - Zeiss Laser Microdissection (Zeiss)
Dissection Microscope
a laser microdissection system based on an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color).
Suitable for the manipulation of biological samples with a UV laser and subsequent contact-free collection of the cut-out material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working widefield imaging system.
Category
Widefield, Microdissection
Location
MPI-CBG, LMF MPI-CBG, room U36
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
2.5x/0.08 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 8.8 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 295 2.5x/0.085 Zeiss |
| Order Number: | 420320-9902 |
5x/0.25 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 12.5 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 296 5x/0.25 Zeiss |
| Order Number: | 420130-9900 |
10x/0.5 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 1.9 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 297 10x/0.5 Zeiss |
| Order Number: | 420140-9900 |
20x/0.4 LD Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0-1.5 |
| Working Distance: | 7.9 mm at cover glass 0.75 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 298 20x/0.4 Zeiss |
| Order Number: | 421350-9970 |
40x/0.6 LD Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0-1.5 |
| Working Distance: | 2.9 mm at cover glass 0.75 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 299 40x/0.6 Zeiss |
| Order Number: | 421360-9970 |
63x/0.75 LCI Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0-1.5 |
| Working Distance: | 1.7 mm at cover glass 0.75 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 300 63x/0.75 Zeiss |
| Order Number: | 421380-9970 |
100x/1.3 EC Plan-Neofluar, Oil, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 301 100x/1.3 Zeiss |
| Order Number: | 420490-9900 |
150x/1.35 Plan-Apochromat, Glycerine, DIC, Zeiss
| Immersion: | Glycerine |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.15-0.19 |
| Working Distance: | 0.17 mm at cover glass 0.16 mm |
| Nomarski Prism: | PA 150x/1,35 III (426968-9000) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 302 150x/1.35 Zeiss |
| Order Number: | 420792-9970 |
Condenser
Fluorescence Filter Cubes
motorized revolver
GFP ()
Dsred ()
Alexa 405 ()
GFP ()
DAPI ()
single cube for DAPI, FITC, Alexa 405
- BP 370/40; BP 474/28; BP 585/35 (BP 350-390; BP 460-488; BP 568-603), excitation filter, triple bandpass, Zeiss
- (DM 395+495+610), triple dichromatic mirror, triple bandpass, Zeiss
- BP 425/46; BP 527/54; LP 615 (BP 402-448; BP 500-554; LP 615), emission filter, triple bandpass, Zeiss
Transmitted Illumination
LED (LED)
Fluorescence Illumination
355nm
Laser Diode
365, 470, 590, 625 nm
Zeiss, Colibri LED, integrated
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
Camera
AxioCam Icc 1, Color CCD camera, dexel size 4.65 µm
| port | top port |
| bit depth | 8 bit |
| sensor size | 6.3 x 4.8 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 15.38 fps |
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | right side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
Extra Photo Manipulation
Additional Information