CFCI-WF 01 ApoTome I (Zeiss)
Widefield System with ApoTome
This microscope is designed for routine work on fixed or living samples. Optical sectioning can be obtained on fixed samples by using the ApoTome (structured illumination). The microscope is also capable for brightfield including contrast techniques like phase contrast, darkfield and polarized light. Furthermore the second camera (colour) allows the imaging of histological stained samples.
Category
Widefield, ApoTome
Location
MTZ, LMF & EMF CFCI, room A.20.055
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.3 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 5.2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MTZ Z_10x_0.3_01 10x/0.3 Zeiss |
| Order Number: | 420340-9901-000 |
20x/0.5 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MTZ Z_20x_0.5_01 20x/0.5 Zeiss |
| Order Number: | 420350-9900-000 |
40x/0.75 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.71 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MTZ Z_40x_0.75_01 40x/0.75 Zeiss |
| Order Number: | 420360-9900-000 |
63x/1.4 Plan-Apochromat, Oil, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MTZ Z_63x_1.4_02 63x/1.4 Zeiss |
| Order Number: | 420780-9900-000 |
20x/0.3 Achrostigmat, Air, Ph1, Zeiss
| Immersion: | Air |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ Z_20x_0.3_01 20x/0.3 Zeiss |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 488049-9901-000)
single cube for DAPI
- G 365, excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
, set 488049-9901-000
GFP (set 489038-9901-000)
single cube for Alexa488/GFP, CFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 495, dichromatic mirror, longpass, Zeiss
- BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
, set 488049-9901-000, set 489038-9901-000
Cy3 (set 489043-9901-000)
single cube for Alexa555/Cy3/RFP, DsRed
- BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
, set 488049-9901-000, set 489038-9901-000, set 489043-9901-000
Transmitted Illumination
Lamp (HAL 100, 12V, 5600 K)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
Camera
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
| Serial number | 112082065 |
Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm
| port | right side port |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
XY Stage
Structured Illumination
ApoTome1, Zeiss
structured illumination for optical sectioning
Computer
Additional Equipment
Pecon Heating Insert P-Set 2000
for dishes and slides
Additional Information