[B CUBE] iSCAT (MIM Facility)
The interferometric scattering (iSCAT) microscope of the MIM Facility at B CUBE is a home-built microscope offering the possibility of label-free detection and tracking of biomolecules. To improve SNR and thus recording speed and localization precision, gold nanoparticles can be used as labels with radii as low as a few nm -- thus providing a huge advantage over the classical dark-field-scattering approach.
Since the move of B CUBE into the new building at Tatzberg 41, we are still wating for the companies to complete the room for the iSCAT setup -- but we are happy to discuss your projects and ideas with you already; just contact us.
Category
Widefield, Interferometric Scattering Detection (iSCAT)
Location
B CUBE, MIM Facility CMCB, room E23
Suitable for Specimen
Microscope Stand
Objectives
100x/1.4 U Plan SApo, Oil, DIC, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.13 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | B CUBE Olympus 100x/1.4 Oil UPLSAPO |
Fluorescence Illumination
Laser 488 nm
Toptica, Diode Laser, 100 mW, manual
Laser 520 nm
Lasertack, Diode Laser, 1 W, manual
Laser 635 nm
Lasertack, Diode Laser, 700 mW, manual
Camera
PhotonFocus MV1-D1024E-160-CL, Monochrome sCMOS camera, dexel size 10.6 µm
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 10.9 x 10.9 mm |
| maximum frame size | 1024 x 1024 dexel |
| maximum full chip frame rate | 150 fps |
Additional Information