Live Cell/TIRF AF6000 CRTD (Leica)
Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal.
Category
Widefield, TIRF
Location
BIOTEC, 2nd floor, LMF CMCB, room 226
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.4 HC PL APO, Air, DIC, Leica
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.22 mm |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 45 10x/0.4 Leica |
| Order Number: | 11506284 |
| Inventory Number: | test345678 |
20x/0.7 HC Plan APO, Air, DIC, Leica
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.59 mm |
| Nomarski Prism: | C |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 17 20x/0.7 Leica |
| Order Number: | 11506166 |
40x/0.85 HC PL APO corr., Air, DIC, Leica
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.24 mm |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 47 40x/0.85 Leica |
| Order Number: | 11506294 |
63x/1.2 HC PL APO lambda blue, Water, DIC, Leica
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.22 mm |
| Nomarski Prism: | D |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 10 63x/1.2 Water Leica corr. |
| Order Number: | 11506213 |
100x/1.46 HC PL APO, Oil, DIC, Leica
| Immersion: | Oil |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 81 mm |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 48 100x/1.46 Oil Leica corr. |
| Order Number: | 11506249 |
Condenser
Fluorescence Filter Cubes
motorized revolver
GFP (set F46-002)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
- LP 495, dichromatic mirror, longpass, Leica
- BP 525/50 (BP 500-550), emission filter, bandpass, AHF
Leica, set F46-002
RFP-T (set F46-002)
single cube for RFP
- BP 552/24 (BP 540-564), excitation filter, bandpass, Leica
- LP 570, dichromatic mirror, longpass, Leica
- BP 605/65 (BP 573-638), emission filter, bandpass, Leica
Leica
Cy5-T (set F46-002)
single cube for Alexa633/Cy5
- BP 635/10 (BP 630-640), excitation filter, bandpass, Leica
- LP 650, dichromatic mirror, longpass, Leica
- BP 720/60 (BP 690-750), emission filter, bandpass, Leica
Leica
B-G-R (DAPI/GFP/RFP) (set F46-002)
triple cube for DAPI/GFP/RFP
- BP 420/30, BP 495/15; BP 570/20 (BP 405-435; BP 488-503; BP 560-580), emission filter, triple bandpass, Leica
- (DM 415+510+590), dichromatic mirror, triple bandpass, Leica
- BP 465/20; BP 530/30; BP 640/40 (BP 455-475; BP 515-545; BP 620-660), emission filter, triple bandpass, Leica
Leica
B-G-R-IR (DAPI/GFP/RFP/Cy5) (set QAX)
quad cube for DAPI/GFP/RFP/Cy5
- BP 402/22, 490/11, 561/10, 636/14 (BP 391-413, 484-495, 556-566, 629-643), excitation filter, quadro bandpass, Leica
- BP 449/58, 518/55, 595/60, 710/100 (BP 420-478, 490-545, 565-625, 660-760), dichromatic mirror, quadro bandpass, Chroma
- BP 451/49, 526/36, 601/31, 707/69 (BP 426-475, 508-544, 585-616, 672-741), emission filter wheel, quadro bandpass, Chroma
Leica, set QAX
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Leica, set Analysator
Additional Filters
DAPI, GFP, RFP, Cy5
- BP 451/49, 526/36, 601/31, 707/69 (BP 426-475, 508-544, 585-616, 672-741), emission filter wheel, quadro bandpass, Chroma
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Leica, Metal halide lamp, 120W, integrated
405nm
Laser Diode, integrated
488nm
Laser DPSS, integrated
561nm
Laser DPSS, integrated
633nm
Laser HeNe, integrated
Camera
Leica DFC 360 FX, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 0.7x |
| bit depth | 12 bit |
| sensor size | 1392 x 1040 pixel |
| maximum frame size | 1392 x 1040 dexel |
| maximum full chip frame rate | 20 - 10 fps |
| Inventory number | 167353000, 71772 |
| Serial number | 287830208 |
Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm
| port | bottom port |
| magnification | 0.7x |
| bit depth | 14 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 35-549 fps |
| Inventory number | 162033000, 71774 |
| Serial number | X-3391 |
XY Stage
Incubation
Computer
Software
Additional Information