LSM 780/FCS inverse BIOTEC (Zeiss)
It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides F(C)CS functionality by the ConfoCor3 module. For FCS please ask Markus Burkhardt.
Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
Category
Laser scanning confocal
Location
BIOTEC, 2nd floor, LMF CMCB, room 221
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.45 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 170 10x/0.45 Zeiss |
| Order Number: | 420640-9900-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 23 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
40x/1.2 C-Achroplan, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm |
| Nomarski Prism: | CA 40/1,2 W; LD CA 40/1,1 W III (426948) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 25 40x/1.2 Water Zeiss |
| Order Number: | 421767-9970-711 |
63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss
| Immersion: | W / Glyc |
| Collar: | immersion correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.17 mm |
| Nomarski Prism: | LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 102 63x/1.3 Imm Zeiss |
| Order Number: | 420882-9970-720 |
5x/0 APO, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 172 APO Calibration Zeiss |
| Order Number: | 420639-9000-700 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 49)
single cube for Alexa 350, DAPI
- BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 49
GFP (set 38)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38
Cy3 (set 43)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 545/25 (BP 533-558), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Zeiss, set 43
Cy5 (set 50)
single cube for Alexa633/Cy5
- BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
Zeiss, set 50
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Zeiss, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, manual
405nm
Laser Diode, integrated
458, 488, 514nm multiline
Laser Argon, integrated
561nm
Laser DPSS, integrated
633nm
Laser HeNe, integrated
XY Stage
Scan Head
Incubation
Computer
Software
Additional Equipment
| MBS | Port |
|---|---|
| Plate | invisible |
| MBS -405 | invisible |
| MBS -445 | invisible |
| MBS T80/R20 | invisible |
| MBS 514/561 | visible |
| MBS 458 | visible |
| MBS 458/514 | visible |
| MBS 458/514/561/633 | visible |
| MBS 488 | visible |
| MBS 488/561 | visible |
| MBS 488/633 | visible |
| MBS 488/561/633 | visible |
| MBS T80/R20 | visible |
| Plate | visible |
Additional Information