LSM 780/FLIM inverse CRTD (Zeiss)
It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
Suitable for the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
Category
Laser scanning confocal
Location
CRTD, basement south, LMF CMCB, room -1.123
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.45 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 154 10x/0.45 Zeiss |
| Order Number: | 420640-9900-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 155 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
40x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm |
| Nomarski Prism: | CA 40/1,2 W; LD CA 40/1,1 W III (426948) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 156 40x/1.2 Water Zeiss |
| Order Number: | 421767-9970-711 |
40x/0.6 LD Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.9 mm at cover glass 0.75 mm |
| Nomarski Prism: | LD PN 40/0,60 II (426955), LD PN 40/0,6 (444505) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 174 LD 40x/0.6 Zeiss |
| Order Number: | 421360-9970-000 |
63x/1.46 alpha Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.1 mm |
| Nomarski Prism: | EC PN 63/1,25 III; CA 63/1,2W III (426946) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 157 63x/1.46 Oil Zeiss |
| Order Number: | 420780-9970-000 |
5x/0 APO, Air, DIC, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 158 APO Calibration Zeiss |
| Order Number: | 420639-9000-700 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 49)
single cube for Alexa 350, DAPI
- BP 347/55 (BP 320-375), excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 49
GFP (set 38)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38
Dsred (set 49005)
single cube for mCherry/TexasRed
- BP 545/30 (BP 530-560), excitation filter, bandpass, Chroma
- LP 570, dichromatic mirror, longpass, Chroma
- BP 620/60 (BP 590-650), emission filter, bandpass, Chroma
Zeiss, set 49005
CFP (set F46-001)
single cube for CFP
- BP 436/20 (BP 426-446), excitation filter, bandpass, AHF
- LP 455, dichromatic mirror, longpass, AHF
- BP 480/40 (BP 460-500), emission filter, bandpass, AHF
Zeiss, set F46-001
GFP/RFP (set F46-001)
double cube for GFP/RFP
- LP 565, dichromatic mirror, longpass, AHF
- BP 525/50 (BP 500-550), emission filter, bandpass, AHF
- BP 617/73 (BP 581-654), emission filter, bandpass, AHF
Zeiss
GFP (set F46-001)
double cube for Alexa488/GFP
- (DM Polarization), dichromatic mirror, longpass, AHF
- BP 525/50 (BP 500-550), emission filter, bandpass, AHF
Zeiss
CFP/YFP (set F46-001)
double cube for CFP/YFP
- LP 498, dichromatic mirror, longpass, AHF
- BP 479/40 (BP 459-499), emission filter, bandpass, AHF
- BP 535/22 (BP 524-546), emission filter, bandpass, AHF
Zeiss
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, manual
440nm pulsed
Laser Diode
458, 488, 514nm multiline
Laser Argon, integrated
561nm
Laser DPSS, integrated
633nm
Laser HeNe, integrated
XY Stage
Scan Head
Incubation
Computer
Software
Additional Equipment
FLIM dual channel unit from B&H
Additional Information