Biopolis Dresden Imaging Platform

DZNE C1 LSM 700 (retired!!!) (Zeiss)

Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Category

Laser scanning confocal

Location

DZNE, LMF DZNE, room 253

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 23 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 24 20x/0.8 Zeiss
Order Number: 420650-9901

40x/0.95 Plan-Apochromat, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.25 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 25 40x/0.95 Zeiss
Order Number: 420660-9970
Condenser

BF, Ph1, Ph2, Ph3, DIC
NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

motorized revolver

GFP (set 38)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Zeiss, set 38

Alexa 546 (set 38)

single cube for Alexa 546, TRITC, Cy3, DsRed

  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Zeiss, set 38

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

Zeiss, set 38, set 488049-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, manual

405nm

Laser Diode, integrated

488nm

Laser DPSS, integrated

555nm

Laser DPSS, integrated

639nm

Laser Diode, integrated
XY Stage

motorized stage

Märzhauser SCAN IM 130x100 DC 1mm with

  • universal disholder
Scan Head

LSM 700

conventional scanner

Internal detectors:

  • 2 confocal PMT (filter)
Additional Information
  • Laser power values
  • See beam path
  • SIP Number 2601000835
  • Contact for Service Raik Staeber raik.staeber@zeiss.com
  • Service Contract Number CZG/1387(2014-2017)
  • DRESDEN concept ID 2038
  • Date of Installation 11.11.2011
  • End of Warranty 04.05.2014