Biopolis Dresden Imaging Platform

DZNE SD1 Spinning Disk (Zeiss)

Fast, "real-time" confocal imaging

Category

Spinning disk

Location

DZNE, LMF DZNE, room 251

Suitable for Specimen
Live samples
Fixed samples
Tissue sections
Suitable for Technique
Multiple positions
Large area tile/mosaic scans
Multi wellplate imaging
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

63x/1.2 C-Apochromat, Water, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 11 63x/1.2 Korr Zeiss
Order Number: 421787-9970

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 12 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 13 20x/0.8 Zeiss
Order Number: 420650-9901

40x/0.95 Plan-Apochromat, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.25 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 14 40x/0.95 Korr Zeiss
Order Number: 420660-9970

5x/0.16 EC Plan-Neofluar, Air, Ph1, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 18.5 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 15 5x/0.16 Zeiss
Order Number: 420331-9911

2.5x/0.08 EC Plan-Neofluar, Air, Ph1, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 8.8 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 16 2.5x/0.085 Zeiss
Order Number: 420320-9901
Condenser

BF, Ph1, Ph2, Ph3, DIC
NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 489038-9901-000

Cy3 (set 489043-9901-000)

single cube for Alexa555/Cy3/RFP, DsRed

  • BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 489043-9901-000

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 489043-9901-000, set 488049-9901-000

Cy5 (set 488049-9901-000)

single cube for Alexa633/Cy5

  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 489043-9901-000, set 488049-9901-000

Additional Filters

Alexa 680, CFP, Cy5, YFP, YFP, Cy5

  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
  • BP 450/50 (BP 425-475), emission filter, bandpass, Zeiss
  • BP 629/62 (BP 598-660), emission filter, bandpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • BP 527/50; BP 645/60 (BP 502-552; BP 615-675), emission filter, double bandpass, Zeiss
Transmitted Illumination

LED (LED)

Fluorescence Illumination

365, 470, 590, 625 nm

Zeiss, Colibri LED, integrated

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated

405nm

Laser Diode, integrated

488nm

Laser OPSL, integrated

561nm

Laser DPSS, integrated

635nm

Laser Diode, integrated
Camera

Zeiss AxioCam 506 mono, Monochrome CCD camera, dexel size 4.54 µm

port SD port
magnification 1x
bit depth 12 bit
sensor size 12.5 x 10 mm
maximum frame size 2752 x 2208 dexel
maximum full chip frame rate 19 fps
Serial number 150130169

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port right side port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 138125751
XY Stage

motorized stage

ZEISS 130x100 STEP (D) with

  • fixed holder for 4 slides
  • multiwell plate holder
  • universal holder K (slides, dishes 24-68mm)
Computer

Computer Model Name

Windows 10 operating system

  • 12 GB RAM
  • Intel Core i5 CPU
  • AMD FirePro V4900 grafic card
  • 32" display
  • 30" display
  • Internal Net ID
Software

Zeiss ZEN Blue 2.3, 2.3

  • Advanced Tiles & Positions
  • Multi channel acquisition
  • Z-control & Autofocus
  • Time Lapse
  • Experiment Designer
  • Extended Focus
  • Image Analysis
  • Software Autofocus
  • Deconvolution basic

OLYMPUS FV10-ASW 4.2, 4.2

  • Z-stack
  • Time Lapse
  • Multi-Position
  • Multi channel acquisition
  • Advanced Tiles & Positions
  • Experiment Designer
Additional Information
  • Laser power values
  • SIP Number 3834004257
  • Contact for Service Raik Staeber raik.staeber@zeiss.com
  • Service Contract Number Po 4500045803
  • DRESDEN concept ID 2039
  • Date of Installation 14.03.2012
  • End of Warranty 04.05.2014