Biopolis Dresden Imaging Platform

DZNE W2 Zeiss ApoTome (Zeiss)

Structured Illumination Microscope

It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.

Category

Widefield, ApoTome

Location

DZNE, LMF DZNE, room 253

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
3D imaging
Timelapse imaging
Microscope Stand

Zeiss Axio Imager.Z1, upright stand
motorized z-focus

Fluorescence Filter Cubes

motorized revolver

Alexa 555 ()

single cube for Alexa 555, TRITC

  • BP 546/12 (BP 540-552), excitation filter, bandpass, Zeiss
  • LP 560, dichromatic mirror, longpass, Zeiss
  • BP 607/65 (BP 575-640), emission filter, bandpass, Zeiss

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 489038-9901-000

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 488049-9901-000

Cy5 (set 488049-9901-000)

single cube for Alexa633/Cy5

  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss

, set 489038-9901-000, set 488049-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Structured Illumination

ApoTome2, Zeiss

structured illumination for optical sectioning

Additional Information
  • SIP Number 3512 000 268
  • Contact for Service Raik Staeber raik.staeber@zeiss.com
  • Service Contract Number PO 4500045803