Biopolis Dresden Imaging Platform

CZ7 - Zeiss LSM 880 Airy upright (Zeiss)

Zeiss LSM 880 upright single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.
Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition.

Category

Laser scanning confocal

Location

MPI-CBG, LMF MPI-CBG, room 038A

Suitable for Specimen
Fixed samples
Tissue sections
Live samples
Suitable for Technique
Spectral unmixing
Large area tile/mosaic scans
Microscope Stand

Zeiss Axio Imager.Z1, upright stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 311 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.5 W Plan-Apochromat, Water, Zeiss

Immersion: Water
Working Distance: 2.6 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 112 20x/0.5 Zeiss
Order Number: 420957-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 313 20x/0.8 Zeiss
Order Number: 420650-9901

25x/0.8 Plan-Neofluar, W / Glyc / Oil, Zeiss

Immersion: W / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: -
Working Distance: 0.21 – 0.17 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 28 25x/0.8 Zeiss
Order Number: 440541
Inventory Number: 4058270000

25x/0.8 LD LCI Plan-Apochromat, Si / Glyc / Oil, DIC, Zeiss

Immersion: Si / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: 0.17
Working Distance: 0.57 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 25/0,8 II (426947)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 312 25x/0.8 Zeiss
Order Number: 420852-9871

40x/1 W Plan-Apochromat, Water, DIC, Zeiss

Immersion: Water
Working Distance: 2.5 mm
Nomarski Prism: W PA 40/1,0 II HC (426967-9020-000 ), W PA 40/1,0 III (426967-9010)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 315 40x/1.0 Zeiss
Order Number: 421462-9900-799

40x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 183 40x/1.2 Zeiss
Order Number: 421767-9971

63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss

Immersion: W / Glyc
Collar: immersion correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.17 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 314 63x/1.3 Zeiss
Order Number: 420882-9970-720

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: PA 63/1,4 HC II (426952), PA 63/1,40 III (426957)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 316 63x/1.4 Zeiss
Order Number: 420782-9900-799
Condenser

H/DIC, Ph1, Ph2, Ph3, DF, DIC I, DIC II, DIC III
NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

motorized revolver

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 488049-9901-000

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 488049-9901-000, set 489038-9901-000

Alexa 546 (set 489038-9901-000)

single cube for Alexa 546, TRITC, Cy3, DsRed

  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

, set 488049-9901-000, set 489038-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120W

Zeiss, Metal halide lamp

405nm

Laser Diode, integrated

458, 477, 488, 514nm multiline

Laser Argon, integrated

561nm

Laser DPSS, integrated

594nm

Laser HeNe, integrated

633nm

Laser HeNe, integrated
XY Stage

motorized stage

PIline Wienecke & Sinske GmbH M-686K008 with

  • universal disholder
  • heating insert P S1
  • holder for 1 slide
Scan Head

LSM 880 AiryScan

conventional scanner

Internal detectors:

  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • 2 confocal PMT (spectral)
  • AiryScan
  • T-PMT

Yokogawa, CSU-X1 5000 dual cam

  • max. spinning disk rotation speed: 1500 - 5000 rpm
  • maximum scanning speed: 1000 fps
  • scan head field of view size: 10 mm x 7 mm
  • physical pinhole diameter: 50 µm
  • physical pinhole spacing: 250 µm
Extra Photo Manipulation

Aperture

Zeiss LSM 880

Photo Manipulation light source:

Laser Diode 405nm

Laser Argon 458, 477, 488, 514nm multiline

Laser DPSS 561nm

Laser HeNe 594 nm

Laser HeNe 633nm

Incubation

stage top incubator

Bachhofer chamber, H. Saur

Feature:

  • Heating

Additional Information
  • Laser power values
  • Contact for Service Zeiss services service.lm.microscopy.de@zeiss.com
  • DRESDEN concept ID 2034
  • Date of Installation 10.11.2015