Biopolis Dresden Imaging Platform

H1 - DeltaVision OMX - SIM !! Retired !! (GE)

Applied Precision Inc Deltavision OMX v4 BLAZE. This microscope is suitable for high resolution imaging of fixed (and live) thin samples down to a resolution of 120nm and very fast widefield imaging. The microscope is equipped with 4x sCMOS cameras, 4 lasers: 405, 488, 568, 642, and set two sets of filters DAPI/GFP/TRITC/CY5 and CFP/YFP/RFP.

Coupled with a DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide.

 

 

Category

Super resolution, Structured Illumination (SIM)

Location

MPI-CBG, LMF MPI-CBG, room 036

Suitable for Specimen
Fixed samples
Molecules at the surface of a cover slip
Tissue sections
Live samples
Suitable for Technique
3D imaging
Microscope Stand

GE OMX V4, inverted stand
manual objective revolver, motorized z-focus

Additional z-focus device: Piezosystem Jena

Objectives

60x/1.42 Plan Apo, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.15 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 54 60x/1.42 Olympus
Order Number: PLAPON60XO
Inventory Number: 40809900
Condenser

BF, DIC
NA 0.55, working distance 27 mm, manual

Fluorescence Filter Cubes

motorized slider

DAPI/GFP/RFP/Cy5 ()

for GFP, DAPI, RFP, Cy5

motorized revolver

CFP/YFP/RFP

for CFP, YFP, RFP, mCherry

Transmitted Illumination

LED (LED)

Fluorescence Illumination

405nm

Laser Diode, integrated

488nm

Laser DPSS, integrated

568nm

Laser DPSS

640nm

Laser DPSS

SSI Lumencor

Lumencor
Camera

PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm

port back port 3
magnification 1.35x
bit depth 15 bit
sensor size 16.6 x 14 mm
maximum frame size 1024 x 1024 dexel

PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm

port back port 2
magnification 1.35x
bit depth 15 bit
sensor size 16.6 x 14 mm
maximum frame size 1024 x 1024 dexel

PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm

port back port 4
magnification 1.35x
bit depth 15 bit
sensor size 16.6 x 14 mm
maximum frame size 1024 x 1024 dexel

PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm

port back port 1
magnification 1.35x
bit depth 15 bit
sensor size 16.6 x 14 mm
maximum frame size 1024 x 1024 dexel
XY Stage

motorized stage

GE OMX with

  • insert for 3.5 cm dishes
  • holder for 1 slide
Hardware Autofocus

GE Ultimate focus

Structured Illumination

OMX, GE

structured illumination for superresolution

Incubation

stage top incubator

GE

Feature:

  • Heating

  • CO2

Additional Information
  • System described by manufacturer
  • See manual
  • Laser power values
  • Contact for Service ordersde@ge.com - Daniel White - Hermann Pfänder
  • DRESDEN concept ID 1268
  • Date of Installation 02.10.2011
  • End of Warranty 31.12.2016
  • Service Visits November 2019