LZ1 - Zeiss Lightsheet Z.1 (Zeiss)
Single Plane Illumination Microscope
The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions.
Suitable for Live imaging in 3D and 4D.
Category
Light sheet
Location
MPI-CBG, LMF MPI-CBG, room 356
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.1, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | MPI-CBG 286 5x/0.1 Zeiss - Illumination |
| Order Number: | 400900-9010 |
5x/0.1, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | MPI-CBG 287 5x/0.1 Zeiss - Illumination |
| Order Number: | 400900-9010 |
10x/0.2, Air, Zeiss
| Immersion: | Air |
| Thread: | M27 |
| Objective ID: | MPI-CBG 385/386 10/0.2 Zeiss - Illumination |
5x/0.16 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 18.5 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 283 5x/0.16 Zeiss - Detection |
| Order Number: | 420330-8210 |
40x/1 Plan-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.5 mm |
| Nomarski Prism: | W PA 40/1,0 III (426967-9010) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 284 40x/1.0 Zeiss - Detection |
| Order Number: | 421462-8210 |
63x/1 Plan-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Working Distance: | 2.1 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 285 63x/1.0 Zeiss - Detection |
| Order Number: | 421480-8210 |
5x/0.16 EC Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 18.5 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 330 5x/0.16 Zeiss - Detection |
| Order Number: | 420330-8210 |
20x/1 Plan Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Thread: | M27 |
| Objective ID: | MPI-CBG 383 20/1.0 W Zeiss - Detection |
Fluorescence Filter Cubes
motorized revolver
Full mirror ()
single cube for Full reflection
- mirror
Zeiss
Universal Blue/Green ()
image splitter cube for DAPI, Alexa488/GFP, Alexa555/Cy3/RFP, Alexa633/Cy5
- LP 490, dichromatic mirror
- LP 505, emission filter
- SP 490, emission filter
Zeiss
GFP/Cy5 ()
image splitter cube for Alexa488/GFP, Alexa633/Cy5
- LP 580, dichromatic mirror, longpass
- BP 525/40, emission filter, bandpass
- LP 660, emission filter, longpass
Zeiss
Universal Green/Red ()
image splitter cube for Alexa488/GFP, Alexa555/Cy3/RFP
- LP 560, dichromatic mirror, longpass
- emission filter
- LP 585, emission filter, longpass
Zeiss
Cy3/Cy5 ()
image splitter cube for Alexa555/Cy3/RFP, Alexa633/Cy5
- BP 595/40, emission filter, bandpass
- LP 640, dichromatic mirror, longpass
- LP 660, emission filter, longpass
Zeiss
CFP/YFP ()
image splitter cube for Alexa555/Cy3/RFP, Alexa633/Cy5
- BP 480/40, emission filter, bandpass
- LP 510, dichromatic mirror, longpass
- BP 545/40, emission filter, bandpass
Zeiss
motorized revolver
Full mirror
single cube for Full reflection
- mirror
Zeiss
BFP/Cy5
image splitter cube for Full reflection
- BP 445/50, emission filter, bandpass
- LP 490, dichromatic mirror
- LP 660, emission filter, longpass
Zeiss
CFP/YFP
image splitter cube for Full reflection
- BP 480/40, emission filter, bandpass
- LP 510, dichromatic mirror, longpass
- BP 545/40, emission filter, bandpass
Zeiss
FITC/TRITC
image splitter cube for Full reflection
- LP 560, dichromatic mirror, longpass
- BP 517/25, emission filter, bandpass
- BP 595/40, emission filter, bandpass
Zeiss
Universal Green/Red
image splitter cube for Alexa488/GFP, Alexa555/Cy3/RFP
- LP 560, dichromatic mirror, longpass
- emission filter
- LP 585, emission filter, longpass
Zeiss
Cy5
image splitter cube for Alexa633/Cy5
- LP 640, dichromatic mirror, longpass
- empty
- LP 640, emission filter, longpass
Zeiss
Transmitted Illumination
LED (LED)
Fluorescence Illumination
405nm
Laser Diode, integrated
445nm
Laser DPSS
488nm
Laser DPSS, integrated
514nm
Laser DPSS
561nm
Laser DPSS, integrated
638nm
Laser DPSS
Camera
PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm
| port | left side port |
| bit depth | 16 bit |
| sensor size | 13.3x13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 30 fps |
PCO edge, Monochrome sCMOS camera, dexel size 6.5 µm
| port | top port |
| bit depth | 16 bit |
| sensor size | 13.3x13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 30 fps |
Incubation
Additional Information