Biopolis Dresden Imaging Platform

MZ2 - Zeiss 2photon inverted (Zeiss)

 Zeiss LSM 780 NLO

Multiphoton Laser Scanning Microscope with a tunable pulsed near-infra-red laser (Chameleon Vision II) for multiphoton excitation. Single/multi-photon point scanning confocal system with two PMTs and one 32-channel GaAsP detector for spectral detection, two non-descanned GaAsP detectors and three PMTs for multi-photon detection, and three transmitted light detectors. Motorized stage for multi-point acquisition.

Category

Laser scanning confocal, Multi photon

Location

MPI-CBG, LMF MPI-CBG, room 456A

Suitable for Specimen
Fixed samples
Live samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Laser cutting/ ablation
Multi wellplate imaging
Photo manipulation (PA/FRAP/FLIP/FLAP/...)
Spectral unmixing
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 118 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 119 20x/0.8 Zeiss
Order Number: 440640-9903
Inventory Number: 4056760000

25x/0.8 LD LCI Plan-Apochromat, W / Glyc / Oil, DIC, Zeiss

Immersion: W / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: 0-0.17
Working Distance: 0.57 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 25/0,8 II (426947)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 120 25x/0.8 Zeiss
Order Number: 420852-9870

40x/1.1 LD C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.62 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 121 40x/1.1 Zeiss
Order Number: 421867-9970
Inventory Number: 4084400000

40x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 122 40x/1.2 Zeiss
Order Number: 421767-9970

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 123 63x/1.4 Zeiss
Order Number: 440760-9905

63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss

Immersion: W / Glyc
Collar: immersion correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.17 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 124 63x/1.3 Zeiss
Order Number: 420882-9970-720
Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

motorized revolver

GFP (set 38)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Zeiss, set 38

Alexa 546 (set 38)

single cube for Alexa 546, TRITC, Cy3, DsRed

  • BP 545/25 (BP 520-570), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Zeiss, set 38

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

Zeiss, set 38, set 488049-9901-000

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, manual

405nm

Laser Diode, integrated

561nm

Laser DPSS, integrated

594nm

Laser HeNe, integrated

633nm

Laser HeNe, integrated

458, 488, 514nm multiline

Laser Argon, manual

680-1080 nm

Coherent, Laser Multiphotonen Chameleon, manual
XY Stage

motorized stage

Märzhauser SCAN IM 130x100 DC 1mm with

  • universal disholder
  • multiwell plate holder
  • heating insert P S1
Hardware Autofocus
Scan Head

LSM 780 NLO

conventional scanner

Internal detectors:

  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • 2 confocal PMT (spectral)
  • T-PMT

External detectors:

  • 2 GaAsP (Zeiss BiG module)
  • 3 PMT (epi-detection)
  • 2 PMT (trans-detection)
Incubation

stage top incubator

Bachhofer chamber, H. Saur

Feature:

  • Heating

Additional Information
  • Laser power values
  • DRESDEN concept ID 1279