SD3 - Zeiss Spinning Disc (Zeiss)
Fast Spinning Disc Confocal (Yokogawa CSU-X1) with DirectFRAP on a Zeiss microscope.The microscope is equiped with two cameras.
Link to pixel size
Category
Spinning disk
Location
MPI-CBG, LMF MPI-CBG, room 038B
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.45 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 199 10x/0.45 Zeiss |
| Order Number: | 420640-9900 |
25x/0.8 LD LCI Plan-Apochromat, W / Glyc / Oil, DIC, Zeiss
| Immersion: | W / Glyc / Oil |
| Collar: | immersion correction |
| Coverslip Thickness: | 0-0.17 |
| Working Distance: | 0.57 mm at cover glass 0.17 mm |
| Nomarski Prism: | LCI PN 25/0,8 II (426947) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 200 25x/0.8 Zeiss |
| Order Number: | 420852-9870 |
| Inventory Number: | 4086710000 |
40x/1.1 LD C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.14-0.19 |
| Working Distance: | 0.62 mm at cover glass 0.17 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 201 40x/1.1 Zeiss |
| Order Number: | 421867-9970 |
40x/1.3 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.21 mm |
| Nomarski Prism: | A, PA 40x/1,30 III (426965) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 04 40x/1.3 Zeiss |
| Order Number: | 420762-9800 |
63x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.14-0.19 |
| Working Distance: | 0.28 mm at cover glass 0.17 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 202 63x/1.2 Zeiss |
| Order Number: | 421787-9970 |
63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss
| Immersion: | W / Glyc |
| Collar: | immersion correction |
| Coverslip Thickness: | 0.15-0.19 |
| Working Distance: | 0.17 mm at cover glass 0.17 mm |
| Nomarski Prism: | LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 203 63x/1.3 Zeiss |
| Order Number: | 420882-9970-720 |
63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Nomarski Prism: | PA 63/1,4 HC II (426952), PA 63/1,40 III (426957) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 204 63x/1.4 Zeiss |
| Order Number: | 420782-9900 |
100x/1.46 alpha Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.11 mm |
| Nomarski Prism: | PA 100/1,46 III (426963) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 205 100x/1.46 Zeiss |
| Order Number: | 420792-9800 |
Condenser
Fluorescence Filter Cubes
motorized revolver
GFP (set 489038-9901-000)
single cube for Alexa488/GFP, CFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 495, dichromatic mirror, longpass, Zeiss
- BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
, set 489038-9901-000
DAPI (set 488049-9901-000)
single cube for DAPI
- G 365, excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
, set 489038-9901-000, set 488049-9901-000
Cy3 (set 489043-9901-000)
single cube for Alexa555/Cy3/RFP, DsRed
- BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
, set 489038-9901-000, set 488049-9901-000, set 489043-9901-000
Cy5 (set 489043-9901-000)
single cube for Alexa633/Cy5
- BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
, set 489038-9901-000, set 488049-9901-000, set 489043-9901-000
Beam combiner 80/20 (set 489076-8113-001)
triple cube for Laser manipulation, CFP, GFP, DsRed
- BP 452/35; BP 525/30; BP 607/45; Transmission 100% (BP 435-470; BP 510-540; BP 585-630; Transmission 100%), triple dichromatic mirror, triple bandpass, Zeiss
Zeiss, set 489076-8113-001
EMPTY (set 489076-8113-001)
empty for through position
- empty
Zeiss, set 489076-8113-001
Additional Filters
DAPI, CFP, DAPI, Alexa 488, Alexa 568, eGFP, DsRed, Alexa 488, eGFP, Alexa 568, Alexa 555, DsRed, mCherry, RFP, Alexa 647, Cy5
- BP 450/50 (BP 425-475), emission filter, bandpass
- LP 458, emission filter, longpass
- BP 527/50; BP 645/60 (BP 502-552; BP 615-675), emission filter, double bandpass
- BP 535/30 (BP 520-550), emission filter, bandpass
- BP 629/62 (BP 598-660), emission filter, bandpass
- BP 690/50 (BP 665-715), emission filter, bandpass
Transmitted Illumination
LED (LED)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
405nm
Laser Diode, integrated
445nm
Laser DPSS
488nm
Laser DPSS, integrated
520nm
Laser DPSS
561nm
Laser DPSS, integrated
639nm
Laser DPSS, integrated
Camera
Photometrics, Monochrome EMCCD camera, dexel size 16 µm
| port | right side port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 33.7 fps |
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
XY Stage
Hardware Autofocus
Zeiss Definite Focus.1
Scan Head
Extra Photo Manipulation
Incubation
Additional Equipment
Objective heater
Additional Information