Biopolis Dresden Imaging Platform

SD4 - Andor Revolution WD Borealis Mosaic (Andor)

Real-Time Confocal Microscope

Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination.

Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time.

Link to pixel sizes

Category

Spinning disk

Location

MPI-CBG, LMF MPI-CBG, room 036

Suitable for Specimen
Live samples
Fixed samples
Suitable for Technique
3D imaging
Confocal
Large area tile/mosaic scans
Microscope Stand

Andor IX 83, inverted stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Prior NanoScanZ

Objectives

10x/0.4 U Plan SApo, Air, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 3.1 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 303 10x/0.4 Olympus
Order Number: UPLSAPO10X2

30x/1.08 U Plan SApo, Silicone, OLYMPUS

Immersion: Silicone
Objective ID: MPI-CBG 304 30x/1.08 Olympus

40x/1.25 U Plan SApo, Silicone, OLYMPUS

Immersion: Silicone
Collar: coverslip correction
Coverslip Thickness: 0.13-0.19
Working Distance: 0.3 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 325 40x/1.25 Olympus
Order Number: UPLSAPO40XS

60x/1.3 U Plan SApo, Silicone, OLYMPUS

Immersion: Silicone
Collar: coverslip correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.3 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 326 60x/1.3 Olympus
Order Number: UPLSAPO60xS2

100x/1.4 U Plan SApo, Oil, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.13 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 225 100x/1.4 Olympus
Order Number: UPLSAPO100XO

150x/1.45 U Apo, Oil, OLYMPUS

Immersion: Oil
Collar: coverslip correction
Coverslip Thickness: 0.13-0.19
Working Distance: 0.08 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 327 150x/1.45 Olympus
Order Number: UAPON150XOTIRF
Condenser

BF, DIC, Ph0
NA 0.55, working distance 27 mm, motorized

Transmitted Illumination

LED (LED)

Fluorescence Illumination

CoolLED pE-4000

CoolLED, LED, integrated

405nm

Laser Diode, integrated

445nm

Laser Diode

488nm

Laser DPSS, integrated

514nm

Laser DPSS

561nm

Laser DPSS, integrated

638nm

Laser DPSS

726nm

Laser DPSS
Camera

Andor iXon Ultra 888, Monochrome EMCCD camera, dexel size 13 µm

port left side port
bit depth 16 bit
sensor size 13.3 x 13.3 mm
maximum frame size 1024 x 1024 dexel
maximum full chip frame rate 26 fps

Andor iXon Ultra 888, Monochrome EMCCD camera, dexel size 13 µm

port left side port
bit depth 16 bit
sensor size 13.3 x 13.3 mm
maximum frame size 1024 x 1024 dexel
maximum full chip frame rate 26 fps
XY Stage

motorized stage

Prior PRO SCAN III

Hardware Autofocus

OLYMPUS IX83 ZDC

Scan Head

Yokogawa, CSU-W1

  • max. spinning disk rotation speed: 4.000 rpm
  • maximum scanning speed: 200 fps
  • scan head field of view size: Variable aperture up to 19 mm x 19 mm
  • physical pinhole diameter: 25µm, 50µm
  • physical pinhole spacing: 250µm
  • brightfield bypass mode
Extra Photo Manipulation

DMD

Andor Mosaic

Photo Manipulation light source:

405nm laser (450mW)

Incubation

cage incubator

Pecon

Feature:

  • CO2

  • Heating

  • Humidity

Additional Information