W1 - Olympus TIRF (OLYMPUS)
Total Internal Reflection Microscope (TIRF) Olympus setup.
Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics.
Category
Widefield, TIRF
Location
MPI-CBG, LMF MPI-CBG, room U32
Suitable for Specimen
Microscope Stand
Objectives
10x/0.4 U Plan SApo, Air, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 3.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 217 10x/0.4 Olympus |
| Order Number: | UPLSAPO10X2 |
100x/0.9 U Plan FL N, Air, OLYMPUS
| Immersion: | Air |
| Working Distance: | 1.0 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 245 100x/0.9 Olympus |
| Order Number: | MPLFLN100X |
100x/1.45 Plan Apo, Oil, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 218 100x/1.45 Olympus |
| Inventory Number: | 4056770000 |
100x/1.7 Apo N, Special Oil, OLYMPUS
| Immersion: | Special Oil |
| Collar: | temperature correction |
| Coverslip Thickness: | 0.15 |
| Working Distance: | 0.08 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 290 100x/1.7 Olympus |
| Order Number: | APON100XHOTIRF |
| Inventory Number: | 4078560000 |
150x/1.45 U Apo, Oil, OLYMPUS
| Immersion: | Oil |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.21 |
| Working Distance: | 0.08 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 219 150x/1.45 Olympus |
| Order Number: | UAPON150XOTIRF |
Condenser
Additional Filters
YFP, Alexa 568, Alexa 594, Alexa 594
- SP 560, dichromatic mirror, shortpass
- LP 590, dichromatic mirror, longpass
- BP 520/15 (BP 512-527), emission filter, bandpass, Semrock
- BP 525/30 (BP 510-540), emission filter, bandpass, Semrock
- BP 617/73, emission filter, bandpass, Semrock
- BP 628/40 (BP 608-648), emission filter, bandpass, Semrock
- (BP 505-545; BP 575-745), emission filter wheel, double bandpass, Chroma
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
X-Cite 120W
X-Cite, Metal halide lamp
561nm
Laser DPSS, integrated
Laser DPSS 491nm
Laser DPSS, 75mW, integrated
MPI-CBG W1 TIRF condenser 491nm/561nm
Laser DPSS, 75mW, integrated
Camera
Andor iXON 897 EMCCD, Monochrome EMCCD camera, dexel size 16 µm
| port | Left side port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 56-11.074 fps |
XY Stage
Incubation
Additional Information