W4 - Zeiss Inverted CCD (Zeiss)
Wide-Field Microscope
Inverted research grade microscope capable many contrasting techniques including Brightfield, Phase Contrast, Darkfield, Differential Interference Contrast (DIC), and Fluorescence
Suitable for fixed samples, fluorescence, transmitted light, z stacks.
Category
Widefield
Location
MPI-CBG, LMF MPI-CBG, room 341
Suitable for Specimen
Microscope Stand
Objectives
1.25x/0.04 Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | - |
| Working Distance: | 4 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 66 1.25x/0.035 Zeiss |
| Order Number: | 440300 |
| Inventory Number: | 4034320000 |
5x/0.15 Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 13.6 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 81 5x/0.15 Zeiss |
| Order Number: | 440321 |
5x/0.15 Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 13.6 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 231 5x/0.15 Zeiss |
| Order Number: | 440321 |
10x/0.45 Plan-Apochromat, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 232 10x/0.45 Zeiss |
| Order Number: | 1063-139 |
| Inventory Number: | 4080250000 |
20x/0.4 LD Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0-1.5 |
| Working Distance: | 7.9 mm at cover glass 0.75 mm |
| Nomarski Prism: | LD PN 20/0,40 II (426954) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 233 20x/0.4 Zeiss |
| Order Number: | 441341-9970 |
20x/0.75 Plan-Apochromat, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.61 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 234 20x/0.75 Zeiss |
| Order Number: | 440649 |
40x/0.75 Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.71 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 235 40x/0.75 Zeiss |
| Order Number: | 440350 |
| Inventory Number: | 4017340000 |
40x/1.3 Plan-Neofluar, Oil, Ph3, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.2 mm |
| Nomarski Prism: | 40x/1.30 oil III (1033-912 ) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 236 40x/1.3 Zeiss |
| Order Number: | 441452 |
| Inventory Number: | 4015730000 |
63x/1.25 Plan-Neofluar, Oil, Ph3, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 238 63x/1.25 Zeiss |
| Order Number: | 440460 |
| Inventory Number: | 4015700000 |
100x/1.3 Plan-Neofluar, Oil, Ph3, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.2 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 239 100x/1.3 Zeiss |
| Order Number: | 1018-595 |
Fluorescence Filter Cubes
0 revolver
DAPI ()
single cube for DAPI, Alexa 350
- BP 388/18 (BP 379-396), excitation filter, bandpass
- LP 414, dichromatic mirror, longpass
- BP 446/62 (BP 415-477), emission filter, bandpass
eGFP ()
single cube for Alexa488/GFP, FITC
- BP 469/40 (BP 449-488), excitation filter, bandpass
- LP 495, dichromatic mirror, longpass
- BP 514/38 (BP 495-532), emission filter, bandpass
Texas red ()
single cube for mCherry/TexasRed
- BP 560/40 (BP 540-580), excitation filter, bandpass
- LP 588, dichromatic mirror, longpass
- BP 631/72 (BP 595-667), emission filter, bandpass
Cy5 ()
single cube for Alexa633/Cy5
- BP 627/46 (BP 604-649), excitation filter, bandpass
- BP 706/84 (BP 664-748), dichromatic mirror, bandpass
- BP 684/46 (BP 661-706), emission filter, bandpass
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HBO
Mercury arc lamp, 100W
Camera
Diagnostic Instrument RT Spot, Monochrome CCD camera, dexel size 7.4 µm
| port | top port |
| bit depth | 8 bit |
| sensor size | 11.1 x 7.9 mm |
| maximum frame size | 1520 x 1080 dexel |
| maximum full chip frame rate | 8.5-19 fps |
Additional Information