CFCI-SD 01 Spinning Disk (OLYMPUS)
The Olympus spinning disc microscope is designed for fast "real-time" confocal imaging of living samples. Furthermore that system is especially designed for mouse intravital microscopy. The microscope is also capable for brightfield including contrast techniques like DIC, darkfield and polarized light.
Category
Spinning disk
Location
MTZ, LMF & EMF CFCI, room A.20.028
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
4x/0.16 U Plan SApo, Air, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | - |
| Working Distance: | 13 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_4x_0,16_01 4x/0.16 Olympus |
| Order Number: | UPLSAPO4X - UIS 2 |
10x/0.4 U Plan SApo, Air, DIC, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 3.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_10x_0,4_01 10x/0.4 Olympus |
| Order Number: | UPLSAPO10X2 - UIS 2 |
20x/0.75 U Plan SApo, Air, DIC, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.6 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_20x_0,75_01 20x/0.75 Olympus |
| Order Number: | UPLSAPO20X - UIS 2 |
40x/0.95 U Plan SApo, Air, DIC, OLYMPUS
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.11-0.23 |
| Working Distance: | 0.18 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_40x_0,95_01 40x/0.95 Olympus |
| Order Number: | UPLSAPO40X - UIS 2 |
60x/1.2 U Plan SApo, Water, DIC, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.21 |
| Working Distance: | 0.28 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_60x_1,2_01 60x/1.2 Olympus |
| Order Number: | UPLSAPO60XW - UIS 2 |
60x/1.42 Plan Apo, Oil, DIC, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.15 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_60x_1.42_01 60x/1.42 Olympus |
| Order Number: | PLAPON60XO - UIS 2 |
30x/1.05 U Plan SApo, Silicone, DIC, OLYMPUS
| Immersion: | Silicone |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.19 |
| Working Distance: | 0.8 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MTZ O_30x_1.05_01 Olympus |
| Order Number: | UPLSAPO30XS |
Condenser
Fluorescence Filter Cubes
revolver
GFP ()
single cube for Alexa488/GFP
- BP 470/20 (BP 460-480), excitation filter
- LP 485, dichromatic mirror
- BP 518/45 (BP 495-540), emission filter
OLYMPUS
RFP ()
single cube for Alexa 568, Cy3, RFP
- BP 545/20 (BP 535-555), excitation filter
- LP 565, dichromatic mirror
- BP 598/55 (BP 570-625), emission filter
OLYMPUS
Cy5 (set 49006)
single cube for Alexa633/Cy5
- BP 620/60 (BP 590-650), excitation filter, bandpass, OLYMPUS
- LP 660, dichromatic mirror, longpass, OLYMPUS
- BP 700/75 (BP 663-738), emission filter, bandpass, OLYMPUS
OLYMPUS, set 49006
Additional Filters
Alexa 405, Alexa 488, Alexa 568, Alexa 647, Alexa 488, GFP, Alexa 488, Alexa 568, GFP, RFP, Alexa 647, Cy5
- BP 440/40; BP 521/21; BP 607/34; BP 700/45 (BP 420-460; BP 511-532; BP 590-624; BP 678-723), emission filter, quadro bandpass, Semrock
- BP 525/50 (BP 500-550), emission filter, bandpass, Semrock
- BP 512/23; BP630/91 (BP 501-524; BP 585-676), emission filter, double bandpass, Semrock
- BP 685/40 (BP 665-705), emission filter, bandpass, Semrock
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HBO
Mercury arc lamp, 100W
405nm
Laser Diode, integrated
488nm
Laser DPSS, integrated
561nm
Laser DPSS, integrated
640nm
Laser Diode, integrated
Camera
Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm
| port | Left side port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 56 fps |
XY Stage
Hardware Autofocus
OLYMPUS IX81-ZDC 2
Scan Head
Incubation
Computer
Additional Equipment
Setup for mouse intravital microscopy:
- Surgery unit with heating lamp
- Induction box for pre-anesthesia
- BioLite Intubation System
- Mouse Ventilation System: Minivent (Hugo Sachs)
- Silicone heating plate with temperature sensor (Friedrich Freek)
Additional Information