Biopolis Dresden Imaging Platform

Combined RNAi and localization for functionally dissecting long noncoding RNAs.

Chakraborty D, Kappei D, Theis M, Nitzsche A, Ding L, Paszkowski-Rogacz M, Surendranath V, Berger N, Schulz H, Saar K, Hubner N, Buchholz F

Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.

Fig.1 taken from Chakraborty et al, 2012.
  • Nat. Methods 2012 Apr 12;9(4):360-2
  • 2012
  • Cell Biology
  • 22327834
  • PubMed

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