Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy.

Weigert M, Subramanian K, Bundschuh ST, Myers EW, Kreysing M

Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.

Journal, Issue, Date: PLoS Comput Biol. 2018 Apr 13;14(4):e1006079
Year: 2018
Research Area: Image Processing
PubMed ID: 29652879
PubMed link: PubMed

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LMF MPI-CBG

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