Layer-by-Layer Assembly of Heparin and Peptide-Polyethylene Glycol Conjugates to Form Hybrid Nanothin Films of Biomatrices.

Thomas AK, Wieduwild R, Zimmermann R, Lin W, Friedrichs J, Bickle M, Fahmy K, Werner C, Zhang Y

We investigated the utility of a heparin/peptide-polyethylene glycol conjugate system to build layer-by-layer (LbL) structures, to assemble tailored multilayer-biomatrices for cell culture. The LbL assembly balances the advantages of polyelectrolyte systems and protein-based systems. Human umbilical vein endothelial cells showed distinct responses to the film thickness and structure; the presence, density, and spatial arrangement of a cell adhesion ligand within the nanothin film; and the pretreatment of the film with morphogens. The LbL technique presents a versatile tool for modifying cell culture substrates with defined and diverse biochemical and structural features, for investigating cell-material interactions.

Journal, Issue, Date: ACS Appl Mater Interfaces. 2018 May 2;10(17):14264-14270
Year: 2018
Research Area: Applied Chemistry
PubMed ID: 29658265
PubMed link: PubMed

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